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. 2019 Mar 15;294(18):7177–7193. doi: 10.1074/jbc.RA118.005659

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© 2019 Pergu et al.

Published under exclusive license by The American Society for Biochemistry and Molecular Biology, Inc.

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Figure 2. — ERp29 interacts with MSec. A, FLAG immunoprecipitates of the MSec-MTAP and empty-MTAP U2OS cell lysates probed for ERp29 by immunoblotting. B, confocal images showing co-localization of MSec (red) with ERp29 (green) in the ER region. Pearson's correlation coefficient (R_coloc) of the whole frame is 0.6766, and that of the selected cell is 0.6866. Scale bar, 20 μm. C, Western blotting of the whole-cell lysate (WCL) and rough endoplasmic reticulum (RER) fractions from U2OS cells probed for the respective proteins as indicated. Shown are ER-positive markers (calnexin and ERp29), cytosolic marker (β-actin), Golgi complex marker (GM130), nuclear marker (histone-3), and cell membrane marker (EGFR).