Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Mar 15;294(18):7177–7193. doi: 10.1074/jbc.RA118.005659

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2019 Pergu et al.

Published under exclusive license by The American Society for Biochemistry and Molecular Biology, Inc.

PMC Copyright notice

Figure 7. — Characterization of the ERp29–MSec interaction. A, SDS-PAGE profiles (Coomassie-stained) of the purified reombinant MSec and ERp29 proteins as indicated. B, purified ERp29 shows no interaction with either purified MSec or MSec from cell lysate. The bottom strips show immunoblots with the respective antibodies as indicated. C, purified MSec does not interact with purified ERp29 but interacts with ERp29 from cell lysate. The bottom strips show immunoblots with the respective antibodies as indicated. D, protease protection assay with proteinase K reveals that MSec is associated with the ER surface toward its cytosolic side. Markers used in immunoblots were calnexin (ER lumen) and Sec61β-cytosolic side (ER surface).