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. 2019 Mar 19;294(18):7169–7176. doi: 10.1074/jbc.AC119.008036

Figure 1.

Figure 1.

Knocking down or ablating eEF2K elevates HSP90 protein levels. A, lysates of A549 cells treated ±IPTG (to induce shRNA expression to knock down eEF2K) or eEF2K+/+ and eEF2K−/− MEFs were subjected to SDS-PAGE followed by WB analysis of the indicated proteins. B and C, quantification of HSP90 levels in A549 cells (B) or MEFs (C) from A. D, eEF2K+/+ and eEF2K−/− MEFs were cultured in low-glucose (5.5 mm) DMEM ± 10 mm 2-DG for 24 h before immunoblot analysis. E, quantification of HSP90 levels from D. P-eEF2, phosphorylated eEF2. *, 0.01 ≤ p < 0.05; **, 0.001 ≤ p < 0.01. Error bars represent S.D.