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AUY922 induces apoptosis in eEF2K−/− MEFs under proteolytic stress. eEF2K+/+ and eEF2K−/− MEFs were treated as in Fig. 3A. A, cell viability and morphology were assessed by microscopic analysis. Scale bars, 500 μm. B, cells were dispersed, stained with annexin V (FITC-A) and PI (PI-A), and then analyzed by flow cytometry. C, quantification of B. ***, p < 0.001. Error bars represent S.D.
