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. 2019 May 3;10:407. doi: 10.3389/fgene.2019.00407

FIGURE 13.

FIGURE 13

Verification of target binding. (A) miR-320-3p putative binding sites in HTATIP2 3′-UTR. Blue letters indicate wild type sites and red letters indicate mutated sites in the pMir-report luciferase reporter vector. Luciferase assays were performed in RAB-9 cells co-transfected with pMir-report-HTATIP2-3′-UTR-WT and miRNA-320-3p mimics, or pMir-HTATIP2-3′-UTR-MUT, and miRNA-320-3p mimics. (B) Putative binding sites for miR-320-3p in LNC_002919. Blue letters indicate wild type sites and red letters indicate mutated sites in the pMir-report luciferase reporter vector. The luciferase assays were performed in RAB-9 cells co-transfected with pMir-LNC_002919-WT and miRNA-320-3p mimics, or pMir-LNC_002919-MUT, and miRNA-320-3p mimics. (C) Putative binding sites for miR-320-3p in novel_circ_0026326. Blue letters indicate wild type sites and red letters indicate mutated sites in the pMir-report luciferase reporter vector. The luciferase assays were performed in RAB-9 cells co-transfected with pMir-novel_circ_0026326-WT and miRNA-320-3p mimics, or pMir-novel_circ_0026326-MUT, and miRNA-320-3p mimics. Error bars indicate the mean ± SD of triplicate experiments. ∗∗P < 0.01.