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Validation of miRNA differential expression results at 90, 130, and 150 days. qRT-PCR validation of miR-128-3p, miR-200a-3p, 27a-3p, and miR-320-3p miRNA expression levels in skin samples between 90, 130, and 150 days. The miRNA expression levels at 130 days and 150 days were normalized to the value at 90 days. Error bars indicate the mean ± SD of triplicate experiments. ∗P < 0.05; ∗∗P < 0.01.
