Figure 30.

Cardiolipin interaction sites on the SecYEG complex. CG simulations of the SecYEG-SecA complex embedded in lipid bilayers with different concentrations of cardiolipins identified specific protein residues responsible for stable interactions with cardiolipins.⁸²⁶ A. CG representation of the complex, with SecY in pink, SecE in tan, SecG in green, and SecA in light blue. Residues involved in the interactions with cardiolipins are highlighted as spheres and colored from yellow to red according to occupancy values determined from the simulations. The newly identified sites 1 and 2 are marked. B. Selected frames from the CG simulations were back-mapped to an atomistic representation to highlight site 1 and 2 with cardiolipin molecules bound. C. Cytoplasmic view of the SecYEG complex in B. SecA is not shown for clarity. Adapted with permission from ref (826). Copyright 2018 Corey et al.