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The absence of DegP protease function stabilizes monomeric LamBG439D at 30°C. Monomeric or trimeric LamB was detected using whole-cell lysates made from strains of the listed genotypes, in ΔdegP or degPS210A yadC::Tn10 backgrounds, and analyzed by SDS-PAGE and immunoblotting. Antibodies that specifically detect monomeric and trimer LamB were used.
