FIG 1.

Genetic context and transcription of the A. tumefaciens ybeY. (A) Atu0358 shares high sequence identity with the YbeY orthologues from S. meliloti, V. cholerae, and E. coli. (B) The ybeY gene cluster in A. tumefaciens consists of the phosphate starvation-inducible protein gene phoH (atu0357), ybeY (atu0358), and the hemolysin gene atu0359. (C) Data from the differential RNA-Seq analysis by Wilms’ et al. (42). The transcription start site of ybeY lies upstream of phoH. cDNA libraries were generated from cultures grown without (–Vir) and with (+Vir) virulence induction by acetosyringone. Screenshots of the results after treatment with terminator 5ʹ-phosphate-dependent exonuclease (TEX) to enrich for primary 5ʹ ends are shown.