FIG 6.
σB influence on IcaC protein levels. (a) Immunodetection of IcaC-tagged protein in the wt and the σB mutants of S. aureus 15981 and 132 at exponential phase. Whole-cell bacterial lysates were subjected to electrophoretic separation in an SDS-12% polyacrylamide gel. The proteins were transferred onto a nitrocellulose membrane and probed with anti-Flag M2 MAb conjugated with peroxidase. Relative quantification of the Flag protein was obtained using the ImageJ program. A Coomassie-stained gel portion is shown as a loading control. (b) Immunodetection of IcaC-tagged protein upon blocking protein biosynthesis in exponentially growing cells. (c) Relative IcaC levels upon blocking protein biosynthesis as an average of the results of two independent experiments; the error bars indicate standard deviations.