TABLE 1.
Strains and plasmids used in this study
| Strain or plasmid | Relevant characteristicsa | Reference |
|---|---|---|
| S. aureus strains | ||
| 15981 | Biofilm-positive clinical strain | 18 |
| ISP479r | ISP479 rsbU-positive strain | 59 |
| 12313 | Biofilm-positive clinical strain | This study |
| 132 | Biofilm-positive clinical strain; MRSA clinical strain | 23 |
| G-6478 | Biofilm-positive clinical strain; MRSA clinical strain | 23 |
| 10833 | Clumping factor-positive variant of Newman D2C | 29 |
| 12313 ΔσB | 12313 with deleted σB gene | This study |
| 10833 ΔσB | 10833 with deleted σB gene | This study |
| ISP479r ΔσB | ISP479r with deleted σB gene | 18 |
| ISP479r Δica | ISP479r with deleted icaADBC operon | This study |
| ISP479r ΔσB Δica | ISP479r σB mutant with deleted icaADBC operon | This study |
| G-6478 ΔσB | G-6478 with deleted σB gene | This study |
| G-6478Δica | G-6478 with deleted icaADBC operon | This study |
| G-6478 ΔσB Δica | G-6478 σB mutant with deleted icaADBC operon | This study |
| 132 ΔσB | 132 with deleted σB gene | This study |
| 132 Δica | 132 with deleted icaADBC operon | 23 |
| 132 ΔσBΔica | 132 σB mutant with deleted icaADBC operon | This study |
| 15981 ΔσB | 15981 σB mutant | 18 |
| 15981 Δica | 15981 with deleted icaADBC operon | 56 |
| 15981 ΔσB Δica | 15981 σB mutant with deleted icaADBC operon | This study |
| 15981 icaC-3×flag | 15981 with a 3×flag-tagged icaC gene | This study |
| 15981 ΔσB icaC-3×flag | 15981 σB mutant with a 3×flag-tagged icaC gene | This study |
| 132 icaC-3×flag | 132 with a 3×flag-tagged icaC gene | This study |
| 132 ΔσB icaC-3×flag | 132 σB mutant with a 3×flag-tagged icaC gene | This study |
| 15981_Pica-gfp | 15981 with pCN52-Pica | This study |
| 15981 ΔσB_Pica-gfp | 15981 σB mutant with pCN52-Pica | This study |
| 132_Pica-gfp | 132 with pCN52-Pica | This study |
| 132 ΔσB_Pica-gfp | 132 σB mutant with pCN52-Pica | This study |
| 15981_Phyp-ica | 15981 constitutively expressing the ica operon | This study |
| 15981 ΔσB_Phyp-ica | 15981 σB mutant constitutively expressing the ica operon | This study |
| 132_Phyper-ica | 132 constitutively expressing the ica operon | This study |
| 132 ΔσB_Phyp-ica | 132 σB mutant constitutively expressing the ica operon | This study |
| 15981 RNAIII | 15981 transformed with plasmid pALC2073 RNAIII | This study |
| 15981 ΔσB RNAIII | 15981 σB transformed with plasmid pALC2073 RNAIII | This study |
| 132 RNAIII | 132 transformed with plasmid pALC2073 RNAIII | 23 |
| 132ΔσB RNAIII | 132 σB transformed with plasmid pALC2073 RNAIII | This study |
| Plasmids | ||
| pMAD | E. coli-S. aureus shuttle vector | 60 |
| pSK9 | Plasmid carrying the σB gene | 16 |
| pHRG | pCN47 derivative plasmid used for posttranscriptional fusions to GFP | This study |
| pCN52-Pica_gfp | Plasmid with GFP expressed under the control of the Pica promoter | This study |
| pCN52-Phyp_gfp | Plasmid with GFP expressed under the control of the Phyper promoter | This study |
| pHRG-5ʹUTRica | Plasmid carrying a posttranscriptional fusion of ica with GFP | This study |
a
MRSA, methicillin-resistant Staphylococcus aureus.