FIG 2.
The J5-c5 transposon mutant induces a proinflammatory response. The amounts of IL-6 secreted by MM6 cells in response to exposure to 106 or 107 intact P. gingivalis 33277 bacteria versus the isogenic ΔPG1587, ΔPG1773, or Δkgp mutant (A) and the J5-c5 transposon mutant (B and C) are shown. The results are means ± standard deviations (SD) for triplicate samples from either one of two independent experiments (A) or from two separate experiments (B and C). Asterisks denote significant differences in amount of IL-6 secreted relative to that for the wild-type 33277 control (P < 0.01 by 2-tailed unpaired t tests).