Table 2.
Differences in the glycopattern of serum proteins between adenocarcinoma groups and healthy control by the lectin microarray analysis based on data of 18 lectins giving significant differences
| Lectin | Specificity | Fold change | ||
|---|---|---|---|---|
| I/II Adenocarcinoma/healthy | III Adenocarcinoma/healthy | IV Adenocarcinoma/healthy | ||
| Jacalin | Galβ1-3GalNAcα-Ser/Thr(T), sialyl-T(ST) | 1.68 | 1.86*** | 2.11** |
| HHL | High-Mannose, Manα1-3Man, Manα1-6Man, Man5-GlcNAc2-Asn | 2.85** | 3.16* | 3.13* |
| PTL-I | GalNAc, GalNAcα-1, 3Gal, GalNAcα-1, 3Galβ-1,3/4Glc | 3.49** | 3.85** | 2.81* |
| SJA | Terminal in GalNAc and Gal | 2.98** | 2.73** | 1.55 |
| AAL | Fucα1-6GlcNAc(core fucose), Fucα1-3 (Galβ1-4) GlcNAc | 2.09*** | 2.71** | 3.57** |
| LTL | Fucα1-2Galβ1-4GlcNAc, Fucα1-3(Galβ1-4) GlcNAc | 0*** | 0*** | 0** |
| GSL-I | αGalNAc,GalNAcα-Ser/Thr (Tn) and αGal | /** | /** | /*** |
| DBA | GalNAcα-Ser/Thr(Tn) and GalNAcα1-3Gal | 4.27* | – | – |
| RCA-120 | β-Gal, Galβ-1, 4GlcNAc(type II), Galβ1-3GlcNAc (type I) | 0.13** | 0.10*** | 0.09** |
| STL | Trimers and tetramers of GlcNAc, core (GlcNAc) of N-glycan, oligosaccharide containing GlcNAc and MurNAc | 0.43** | 0.45** | 0.44** |
| BS-I | αGal and αGalNAc | /*** | /*** | /** |
| ConA | High-Mannose, Manα1-6(Manα1-3)Man, terminal GlcNAc | 0.35*** | 0.35*** | 0.36*** |
| DSA | β-D-GlcNAc, (GlcNAcβ1-4)n, Galβ1-4GlcNAc | 3.77*** | 3.62*** | 3.49*** |
| SBA | Terminal GalNAc (especially GalNAcα1-3Gal) | 11.75*** | 13.96*** | 16.31*** |
| PSA | α-D-Man, Fucα-1, 6GlcNAc (core fucose), α-D-Glc | 9.36** | 5.92*** | 6.20** |
| UEA-I | Fucα1-2Galβ1-4GlcNAc | 7.99 | 5.29*** | 4.46** |
| MAL-I | Galβ-1,4GlcNAc, Siaα2-3Gal, Galβ1-3GlcNAc, Siaα2-3 | /** | /*** | /*** |
| BPL | Galβ1-3GalNAc, terminal GalNAc | /** | /* | /* |
–, no significant difference; /, the denominator of the fold change was zero
*P < 0.05, **P < 0.01, ***P < 0.001