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. 2019 May 10;12:261. doi: 10.1186/s13104-019-4301-5

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© The Author(s) 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Example of amplification plot for approach number 3. The amplification of miR-16, miR-92a and miR-126 when using platelet-poor plasma (PPP) direct as template for reverse transcription compared to analysis using miRNA purified from the same PPP (RNA). The reverse transcription and qPCR were performed in the same runs