Fig. 6.

MALAT1 promoted tumor growth by regulating the expression of miR-141-3p and SMAD5. The MALAT1 was stably knocked down and overexpressed in HepG2 cells, and stably cell lines were established. A xenograft model was established by the stably established cell lines. a, b Representative images of tumors after indicated treatment. The changes in tumor volume were monitored and shown (n = 5 per group). c The RNA level of miR-142-3p, vimentin and E-cadherin in the tumor tissues of the indicated group was assessed by Q-PCR. d The protein expression of vimentin and E-cadherin in the tumor tissues was evaluated by Western blot. The densitometry plot of results from the blots is shown. The relative expression levels were normalized to GAPDH. e, f The protein expression of Ki-67 and SMAD5 in the tumor tissues was analyzed by immunohistochemistry. Scale bar, 50 μm. Data represent three independent experiments (mean and SEM of triplicate samples). *P < 0.05, **P < 0.01. ***P < 0.001. Glyceraldehyde 3-phosphate dehydrogenase, GAPDH; SEM, standard error of the mean