Fig. 2. X-band EPR and FTIR spectroscopic characterization of artificially matured CaHydF, [2Fe]adt-CaHydF. (A) EPR spectrum recorded on as-prepared [2Fe]adt-CaHydF (0.2 mM); (B) FTIR spectrum recorded on as-prepared [2Fe]adt-CaHydF (2.5 mM); (C) EPR spectrum recorded on NaDT reduced [2Fe]adt-CaHydF (0.2 mM, 4 mM NaDT); (D) FTIR spectrum recorded on NaDT reduced [2Fe]adt-CaHydF (2.5 mM, 50 mM NaDT); (E) EPR spectrum recorded on thionine oxidized [2Fe]adt-CaHydF (0.25 mM, 2.5 mM thioinine); (F) FTIR spectrum recorded on thionine oxidized [2Fe]adt-CaHydF (2.5 mM, 25 mM thioinine). All samples were prepared in 25 mM Tris-HCl pH 8.0 50 mM KCl. EPR spectra were recorded at a microwave power of 1 mW, at 10 K, microwave frequency: 9.28 GHz.
