Figure 6.

3D co-cultured multicellular tumor spheroids penetration. (A) The culture and penetration experiment procedure of 3D co-cultured multicellular tumor spheroids. (B) Images of 3D co-culture multicellular tumor spheroids by SEM and CLSM. RAW264.7 cells were labeled with DiI. Scale bar =100 µm. (C) Penetration of coumarin-6-loaded liposomes in 3D co-cultured multicellular tumor spheroids. Scale bar =500 µm.

Abbreviations: Lipo, liposomes; PEG-Lipo, PEGylated liposomes; Man-PEG-Lipo, mannosylated liposomes; 3D, three-dimensional; SEM, scanning electron microscope; CLSM, confocal laser scanning microscopy.