FIGURE 5.

Neurotransmitters, neuromodulators and polyamine biosynthesis at 72 h after TBI in the developing brain. (A) Volcano plot showing FC on x-axis and FDR-adjusted P-values on y-axis. Black vertical and horizontal lines reflect the filtering criteria (FC = 1.0 and FDR corrected P-value < 0.05). Green or red dots represent metabolites that are significantly increased or decreased after TBI, respectively. 13% of the metabolites showed a significant change between sham and TBI. Table on right shows metabolites that are significantly different as denoted by respective numbers in the figure on the left panel. (B,C) PLS-DA analysis of the metabolite subgroup showing distinct class separation between sham and TBI. (D) PLS-DA Model validation analysis and the predictive accuracy indicate good accuracy of Q² = 0.41, 0.68 and 0.86 for the three components, respectively.