Skip to main content
. 2019 May 3;9:351. doi: 10.3389/fonc.2019.00351

Figure 2.

Figure 2

Presence of tight junction proteins in MDCKII/MDR1 monolayer. Representative confocal images of ZO-1 (left) and occludin (right) immunofluorescence (green) showing characteristic tight junction localization between cells of the monolayer. Nuclei are counterstained with Hoechst (blue). Images were acquired with a 40X objective on the Thermo Scientific CX7 CellInsight and processed with ImageJ using the smoothing filter and rolling ball background subtraction (250 pixel radius). Scale bars = 50 μm.