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Concentration-dependent cytotoxicity of LN-229 spheroids. LN-229 viability after 48 h of direct exposure to varying concentrations of cisplatin (white triangles) and piperlongumine (gray squares). Normalized viability was calculated by comparing luminescent signal in the presence of drug to that of buffer plus vehicle controls. Mean ± SD. N = 12 wells per concentration from two independent studies.
