Table 3.
Prediction of signal peptides physico-chemical properties
| Signal Peptides |
Amino Acid
Length |
MW | pI | Net Positive Charge | Aliphatic Index | GRAVY |
Instability Index
Without protein |
Instability index with protein |
Solubility
(Probability) |
|---|---|---|---|---|---|---|---|---|---|
| PPB_ECOLI | 21 | 2256.82 | 10.00 | 2 | 139.52 | 0.971 | Unstable (56.02) | Unstable (19.61) | Soluble(0.661) |
| PHOE_ECOLI | 21 | 2104.59 | 10.00 | 2 | 130.00 | 1.195 | Stable (1.44) | Stable (15.42) | Soluble(0.688) |
| OMPA_ECOLI | 21 | 2046.50 | 10.00 | 2 | 121.43 | 1.295 | Stable (9.52) | Stable (16.04) | Soluble(0.697) |
| OMPF_ECOLI | 22 | 2266.83 | 11.00 | 2 | 150.91 | 1.259 | Unstable (67.18) | Stable (20.64) | Soluble(0.678) |
| OMPT_ECOLI | 20 | 2102.61 | 11.00 | 2 | 146.50 | 1.290 | Stable (2.62) | Stable (15.55) | Soluble(0.672) |
| OMPC_ECOLI | 21 | 2078.63 | 10.00 | 2 | 171.90 | 1.552 | Stable (14.37) | Stable (16.41) | Soluble(0.695) |
| ELBH_ECOLX | 21 | 2358.84 | 9.10 | 2 | 111.43 | 0.695 | Stable (26.85) | Stable (17.37) | Soluble(0.608) |
| LAMB_ECOLI | 25 | 2545.22 | 11.00 | 2 | 125.20 | 1.332 | Unstable (42.97) | Stable (18.96) | Soluble(0.668) |
| MALE_ECOLI | 26 | 2698.34 | 11.17 | 3 | 113.08 | 1.012 | Stable (2.85) | Stable (15.30) | Soluble(0.657) |
| DSBA_ECOLI | 19 | 1990.48 | 10.00 | 2 | 144.21 | 1.416 | Stable (11.50) | Stable (16.22) | Soluble(0.662) |
| DGAL_ECOLI | 23 | 2362.89 | 10.00 | 2 | 102.17 | 0.952 | Stable (14.15) | Stable (16.38) | Soluble(0.668) |
| TORT_ECOLI | 18 | 2111.72 | 9.50 | 1 | 173.33 | 2.061 | Stable (26.66) | Stable (17.25) | Soluble(0.640) |
| TOLB_ECOLI | 21 | 2371.92 | 11.00 | 2 | 139.52 | 1.219 | Unstable (43.26) | Stable (18.63) | Soluble(0.618) |
| HSTI_ECOLX | 23 | 2552.09 | 9.70 | 2 | 102.17 | 1.026 | Stable (32.43) | Stable (17.91) | Soluble(0.608) |
| BLAT_ECOLX | 23 | 2626.22 | 8.02 | 1 | 110.43 | 1.539 | Unstable (56.40) | Stable (19.91) | Soluble(0.573) |
| ASPG2_ECOLI | 22 | 2274.76 | 8.35 | 1 | 93.64 | 1.136 | Stable (-1.15) | Stable (15.16) | Soluble(0.683) |
| CEXE_ECOLX | 19 | 1979.51 | 9.70 | 2 | 154.21 | 1.411 | Stable (29.75) | Stable (17.50) | Soluble(0.678) |
| PTRA_ECOLI | 23 | 2613.20 | 11.00 | 2 | 131.74 | 0.857 | Unstable (51.93) | Stable (19.54) | Soluble(0.604) |
| FKBA_ECOLI | 25 | 2676.31 | 10.00 | 2 | 121.20 | 1.212 | Stable (14.37) | Stable (16.38) | Soluble(0.649) |
| LPP_ECOLI | 20 | 1956.46 | 10.00 | 2 | 161.00 | 1.400 | Stable (10.64) | Stable (16.14) | Soluble(0.707) |
| THIB_ECOLI | 18 | 1974.60 | 8.89 | 2 | 157.22 | 1.589 | Unstable (65.64) | Stable (19.85) | Soluble(0.646) |
| TAUA_ECOLI | 22 | 2308.72 | 9.50 | 1 | 120.45 | 1.055 | Stable(34.41) | Stable (18.01) | Soluble(0.659) |
| PSPE_ECOLI | 19 | 2065.63 | 10.00 | 2 | 148.95 | 1.711 | Stable (17.37) | Stable (16.64) | Soluble(0.643) |
| PPA_ECOLI | 22 | 2384.99 | 8.50 | 1 | 155.45 | 1.405 | Unstable (53.16) | Stable (19.52) | Soluble(0.645) |
| OMPP_ECOLI | 23 | 2406.88 | 5.75 | 0 | 114.78 | 0.904 | Unstable (44.47) | Stable (18.91) | Soluble(0.697) |
| DRAA_ECOLX | 21 | 2135.63 | 10.00 | 2 | 98.10 | 1.162 | Stable (16.49) | Stable (16.57) | Soluble(0.674) |
| OMPW_ECOLI | 21 | 2093.55 | 10.00 | 2 | 125.71 | 1.210 | Stable (1.44) | Stable (15.42) | Soluble(0.694) |
| PBP7_ECOLI | 25 | 2705.36 | 11.00 | 2 | 117.20 | 1.228 | Unstable (57.99) | Stable (20.32) | Soluble(0.611) |
| NIKA_ECOLI | 22 | 2434.99 | 10.35 | 2 | 137.73 | 1.350 | Unstable (60.45) | Stable (20.10) | Soluble(0.634) |
| LPTA_ECOLI | 27 | 2849.47 | 10.30 | 3 | 130.37 | 0.881 | Stable (17.32) | Stable (16.65) | Soluble(0.628) |
| SUBF_BACSU | 30 | 3145.80 | 12.02 | 5 | 117.00 | 0.497 | Stable (20.75) | Stable (17.02) | Soluble(0.634) |
| CWBA_BACSU | 25 | 2649.35 | 8.89 | 2 | 160.00 | 1.596 | Unstable (42.97) | Stable (18.96) | Soluble(0.634) |
| QOX2_BACSU | 26 | 2843.68 | 11.00 | 2 | 198.46 | 2.242 | Stable (8.20) | Stable (15.80) | Soluble(0.646) |
| CHIS_BACSU | 35 | 4094.03 | 9.52 | 2 | 83.71 | 0.800 | Stable (17.79) | Stable (16.73) | Soluble(0.577) |
| SACB_BACAM | 29 | 3008.61 | 10.30 | 3 | 107.93 | 0.710 | Stable (26.18) | Stable (17.57) | Soluble(0.642) |
| BLAC_BACSU | 36 | 3875.66 | 9.30 | 3 | 92.22 | 0.628 | Stable (4.18) | Stable (15.03) | Soluble(0.575) |
| GUB_BACAM | 25 | 2640.30 | 9.50 | 2 | 148.00 | 1.508 | Unstable (45.44) | Stable (19.18) | Soluble(0.657) |
| CDGT_BACST | 31 | 3562.25 | 8.50 | 1 | 116.13 | 0.887 | Unstable (46.86) | Stable (19.90) | Soluble(0.632) |
| THER_BACST | 25 | 2414.95 | 11.00 | 2 | 121.60 | 1.100 | Stable (33.02) | Stable (18.06) | Soluble(0.678) |
| BLAC_BACLI | 26 | 2810.54 | 10.04 | 4 | 131.54 | 1.192 | Stable (15.30) | Stable (16.46) | Soluble(0.607) |
| AMY_BACLI | 29 | 3299.07 | 11.10 | 4 | 141.72 | 0.752 | Unstable (50.51) | Stable (20.08) | Soluble(0.577) |
MW (molecular weight), pI (isoelectric point), Instability index, GRAVY (grand average of hydropathicity). Proteins with instability index more than 40 were considered as unstable.