Figure 5.

miR-497-5p targets DLX6-AS1 and negatively correlates with DLX6-AS1 expression.

Notes: (A) The possible target of DLX6-AS1 was predicted by starBase v2.0. The DLX6-AS1 binds the sites in the 3′-UTR of miR-497-5p. (B) The mRNA expression of miR-497-5p was detected by qRT-PCR. miR-497-5p was downregulated in PC tissues compared with adjacent normal tissues. (C) miR-497-5p was negatively correlated with DLX6-AS1 expression. (D) The binding of miR-497-5p and DLX6-AS1 was validated by RNA pull-down assay. DLX6-AS1 overexpression vector presented a higher miR-497-5p enrichment than that of the control. (E) The binding of miR-497-5p and DLX6-AS1 was validated by dual-luciferase reporter assay. Unlike negative control (NC) mimics, miR-497-5p mimics reduced the luciferase activity of wild-type (WT) DLX6-AS1 reporter vector but not that of mutant (MUT) one. (F) Overexpression of DLX6-AS1 decreased miR-497-5p level, whereas knockdown of DLX6-AS1 increased miR-497-5p level. Data are presented as mean ± SD. ***P<0.001.

Abbreviations: PC, pancreatic cancer; qRT-PCR, quantitative reverse transcription PCR.