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. 2019 May 3;10:468. doi: 10.3389/fphar.2019.00468

FIGURE 1.

FIGURE 1

Repeated treatment with E-52862 reversed mechanical hypersensitivity associated to osteoarthritis pain. (A) Mice received an intra-knee injection of MIA or saline and were treated with vehicle or E-52862 (20 mg/kg) from day 1 until the end of the experiment (day 25). Mechanical thresholds were assessed with the von Frey under basal conditions and on days 5 and 19 after the intra-articular injection, and gait was analyzed with the Catwalk test on days 6 and 12. (B) MIA-injected mice treated with vehicle showed a decrease on mechanical thresholds that was reversed in E-52862-treated mice. Catwalk analysis revealed a decrease of the ratio (right hind/left hind paws) of print area (C) and maximal contact area (D) in mice injected with MIA and treated with vehicle. This alteration was reversed in mice receiving E-52862. Swing (E) and duty cycle (F) were not significantly altered by the intra-knee injection. Data are expressed as mean ± SEM (n = 6–8 animals per group). ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001 vs. Saline-vehicle, ##p < 0.01, ###p < 0.001 for MIA-vehicle vs. MIA-E-52862 (3-way repeated measures ANOVA followed by Fisher Least Significant Difference test). MIA, monosodium iodoacetate; SEM, standard error of the mean; RH, right hind; LH, left hind.