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. 2019 May 3;10:468. doi: 10.3389/fphar.2019.00468

FIGURE 5.

FIGURE 5

E-52862 decreased pain-induced microgliosis in the medial prefrontal cortex. Microglial cells were detected in the prelimbic and infralimbic areas of the medial prefrontal cortex (mPFC) of saline and MIA injected mice after the repeated treatment with vehicle or the σ1R antagonist E-52862 (20 mg/kg, twice daily during 25 days). Mice with osteoarthritis pain treated with vehicle showed an increased density of microglial cells in the prelimbic (A) and the infralimbic areas (D). This microgliosis was normalized by the treatment with E-52862 (A,D). MIA-injected mice revealed larger perimeters of the soma of microglial cells in the infralimbic (E), but not in the prelimbic area (B). Treatment with E-52862 did not modify this alteration (E). (C,F) Representative images of all groups are shown. Data are expressed as the mean ± SEM (n = 7 animals per group). Scale bar: 100 μm. ∗p < 0.05, ∗∗∗p < 0.001 for saline vs. MIA, ##p < 0.01 for vehicle vs. E-52862 (two-way ANOVA). MIA, monosodium iodoacetate; SEM, standard error of the mean.