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. Author manuscript; available in PMC: 2019 Jul 1.
Published in final edited form as: Vet Clin North Am Small Anim Pract. 2019 Apr 6;49(4):671–686. doi: 10.1016/j.cvsm.2019.02.007

Figure 4.

Figure 4.

Specificity of the antibody response to OspC in infected dogs and horses. Recombinant OspC proteins (types I, F and T) with or without the putative C10 epitope were produced, purified and transferred onto membranes for immunoblot analysis. The membranes were screened with serum from an infected dog (ID #: TF1286; top panel) or an infected horse (ID #: 1026; bottom panel) and IgG binding to each protein assessed using the appropriate secondary antibody and chemiluminescence. Note that while antibody to OspC was detected, the antibody was not cross reactive with the different OspC types. In addition, deletion of the C10 motif from each protein had no discernable impact on the level of antibody binding.