FIGURE 1.

Construction and identification of stable cell lines. (A) The HSC-T6 cell line was transfected with a lentivirus containing NOX4 shRNA. Cells were observed under an optical microscope (left) and a fluorescence (GFP) microscope (right) in the same field of vision. (B) RT-qPCR analysis of Nox4 mRNA in the NOX4 shRNA stable cell line after TGF-β1 treatment (10 ng/mL) for 48 h. (C) WB analysis of the NOX4 protein levels in the NOX4i group. (D) The HSC-T6 cell line was transfected with a lentivirus containing RhoA shRNA. Cells were observed under an optical microscope (left) and a fluorescence microscope (right) in the same field of vision. (E) RT-qPCR analysis of Rhoa mRNA in the Rhoa shRNA stable cell line. (F) WB analysis of the RhoA protein levels in the RhoAi group. (G) The HSC-T6 cell line was transfected with a Nox4 overexpression lentivirus. Cells were observed under an optical microscope (left) and a fluorescence microscope (right) in the same field of vision. (H) RT-qPCR analysis of Nox4 mRNA in the NOX4 overexpression stable cell line. (I) WB analysis of the NOX4 protein levels in the NOX4^{GV 358Lv} group. Original magnification, 200×. The data are presented as the means ± SEMs of three replicates. ^∗p < 0.05 versus the CON group (lentivirus containing a scramble sequence).