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. 2019 May 3;10:431. doi: 10.3389/fphar.2019.00431

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Copyright © 2019 Huang, Gan, Luo, Wan, Chen, Wang, Li and Zhu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The regulatory effects of NOX4 and RhoA as well as the UA intervention effects on the migration and invasion of HSCs after TGF-β1 treatment (10 ng/mL) for 48 h. (A) Cell proliferation detected by the MTS assay. (B,C) Cell apoptosis levels detected by the TUNEL assay. Black arrows: typical apoptotic cells. Original magnification, 200×. Percentage of apoptotic cells = apoptotic cells/cell count per high-power field; 10 different fields of vision were randomly selected in each group. (D,E) Cell scratch assay. Original magnification, 100×. (F,H) Cell invasion detected by the Transwell invasion system. Original magnification, 200×; (G) MTS indirect cell count curve. r = 0.9959. The data are presented as the means ± SEMs of three replicates. ^∗p < 0.05, ^∗∗p < 0.01 versus the CON group; ^#p < 0.05, ^##p < 0.01 versus the NOX4^{GV 358Lv} group. ∆p < 0.05 versus the NOX4i group.