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. 2019 May 3;10:431. doi: 10.3389/fphar.2019.00431

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Copyright © 2019 Huang, Gan, Luo, Wan, Chen, Wang, Li and Zhu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The regulatory effects of NOX4 and RhoA as well as the UA intervention effects on the F-actin polymerization levels in each group after TGF-β1 treatment (10 ng/mL) for 48 h. (A,B) WB analysis of F-actin in each group. The data are presented as the means ± SEMs of three replicates. ^∗p < 0.05, ^∗∗p < 0.01 versus the CON group; ^#p < 0.05, ^##p < 0.01 versus the NOX4^{GV 358Lv} group. (C,D) F-actin immunofluorescence staining in each group. Mean integral optical density (IOD) values were measured by Image Pro Plus 6.0 software; 10 different fields of vision were randomly selected. Original magnification, 400×.