Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 May 3;10:431. doi: 10.3389/fphar.2019.00431

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2019 Huang, Gan, Luo, Wan, Chen, Wang, Li and Zhu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

Construction and identification of NOX4^{GV 358Lv}-RhoA shRNA stable cell lines. (A) The NOX4^{GV 358Lv} cell line was transfected with a lentivirus containing RhoA shRNA. Cells were observed under an optical microscope (left) and a fluorescence microscope (right) in the same field of vision. Original magnification, 200×; (B) RT-qPCR analysis of Nox4 mRNA in the NOX4 shRNA stable cell line. (C) WB analysis of the NOX4 protein levels in the NOX4^{GV 358Lv}-RhoAi group. (D) Cell proliferation detected by the MTS assay. (E,F) Cell apoptosis levels detected by the TUNEL assay. Black arrows: typical apoptotic cells. Percentage of apoptotic cells = apoptotic cells/cell count per high-power field; 10 different fields of vision were randomly selected from each group. Original magnification, 200×; (G,H) Cell scratch assay. Original magnification, 100×. The data are presented as the means ± SEMs of three replicates. ^∗p < 0.05, versus the NOX4^{GV 358Lv}-con group; ^#p < 0.05, versus the NOX4^{GV 358Lv}-RhoAi group.