Skip to main content
. 2019 May 3;10:422. doi: 10.3389/fgene.2019.00422

FIGURE 2.

FIGURE 2

TALE treatment affects the binding of epigenetic modification enzymes to and alters chromatin modifications in gene promoters. (A–D) Chromatin immunoprecipitation (ChIP) assays of binding of DNMT1 (A), EZH2 (B), LSD1 (C), and HDAC1 (D) to promoter regions of CDH1, CDKN1A, NEUROD1, and TUBB3 in HepG2 cells that were treated with DMSO or TALE. (E–I) ChIP assays of changes of histone modifications H3K27me3 (E), H3K27ac (F), H3K9ac (G), H3K4me1 (H), and DNA methylation 5-mC (I) in promoters of CDH1, CDKN1A, NEUROD1, and TUBB3 in HepG2 cells treated with DMSO or TALE. In each panel, the numbers of the vertical axis are arbitrary units indicating relative enrichment of chromatin fragments precipitated by an antibody; the numbers of the horizontal axis represent different amplified regions of a promoter, as described in Materials and Methods. Significance of changes in enrichment of chromatin fragments from cells with or without TALE treatment was calculated for experiments in triplicate using Student’s t-test. Data are shown as mean ± SEM. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001.