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. 2019 May 3;10:422. doi: 10.3389/fgene.2019.00422

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Copyright © 2019 Lei, Chen, Zhang, Yang, Xu, Cao, Zhang and Cao.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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EZH2 knockdown repressed TGFβ and Wnt pathway target gene activation and reduced binding of chromatin modification enzymes to gene promoters. (A,B) Effect of EZH2 knockdown on the activity of TGFβ (A) or Wnt (B) responsive luciferase reporter genes. Relative luciferase activities are shown as mean ± SEM, and significance was calculated for four experiments using Student’s t-test. ^∗∗∗p < 0.001. (C–E) ChIP assay of binding of LSD1 (C), HDAC1 (D), and DNMT1 (E) to different promoter regions of neuronal genes in cells without (shCtrl) and with EZH2 knockdown (shEZH2). Relative chromatin binding enrichments are shown as mean ± SEM, and significance was calculated for four experiments using Student’s t-test. ^∗∗∗p < 0.001.