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. 2019 May 3;10:464. doi: 10.3389/fphys.2019.00464

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Copyright © 2019 Liu, Zhao, Zhang, Li, Shen, Liu, Jiang, Luo, Wang, Che and Xu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Cyr61 was low expressed in renal fibroblasts at 3 days after IR. Bioinformatics was used to preprocess the GSE62732 gene chip data and calculate the difference of Cyr61 expression. The RNA degradation curve (A) and NUSE box plot (B) were used to screen unqualified samples (the slope of the RNA degradation curve is significantly larger or the NUSE value is more than 1.05). (C) RMA standardization was used for subsequent analysis. (D) The expressions of Cyr61 gene in control and IR fibroblasts. ^∗P < 0.05.