FIGURE 5.

Cyr61 inhibited the fibrosis phenotype of normal and activated renal fibroblasts. NRK-49F cells were grouped into four groups: control group (null vector transfection), activated group (5 μg/L TGF-β1), Cyr61⁺ group (over-expression vector transfection), and Cyr61⁺ activated group (over-expression vector transfection + 5 μg/L TGF-β1). The transcriptions of Cyr61, fibrosis factors (Col1α1 and Col3α1) and anti-fibrosis factors (MMP9 and MMP13) were detected by real time PCR (A–C). The expression of Cyr61, Col3, and MMP9 protein was detected by western blotting (D). Relative protein levels based on western blot results (E). ^∗P < 0.05, ^∗∗P < 0.01, and ^∗∗∗P < 0.001 vs. control group; ^#P < 0.05, ^##P < 0.01, and ^###P < 0.001 vs. activated group.