Table 1.

Primer set for VP7 genotyping.

	Primer	5′-Sequence-3′	Position	Product size (bp)
1st PCR	VP7 C-040F	CTCCTTTTAATGTATGGTATTGAATATACC	40–69
	VP7 C-941R	GTATAAAANACTTGCCACCATTTTTTCCA	913–941	902
2nd PCR	VP7 C-0932R	ACTTGCCACCATTTTTTCCA	913–932
	G1-297F	GTATTATCCAACTGAAGCAAGTAC	297–320	636
	G2-401F	TTAAAGACTACAATGATATTACTACATT	401–428	532
	G3-809F	CAAGGGAAAACGTRGCAGTTA	809–829	124
	G3e-757F	CTAGATGTTACTACGGCTAC	757–776	176
	G4-478F	TTCGCTTCTGGTGAGGAGTTG	478–498	455
	G8-179F	TTACRCCATTTGTAAATTCACAG	179–201	754
	G9-606F	GATGGGACARTCTTGTACCATA	606–627	327
	G12-669F	TACRACAACCGACGTCACA	669–687	264
Primer set for VP4 genotyping
1st PCR	VP4 C Ad-F	GGGGGCTATAAAATGGCTTC	1–17
	VP4 C-819r	CTCTATTATATTGCATTTCTTTCCA	790–814	822
2nd PCR	VP4 C Ad-F	GGGGGCTATAAAATGGCTTC	1–17
	P4-361r	GCCTATTTGTTTGACTRACATG	340–361	364
	P6-613r	CATGTATTACAGTTTCTACTTCAG	590–613	616
	P8-447r	CTGCYTCTAAACATTTCTAAAAAC	423–447	450
	P9-193r	GCACTAATGTAGAATCAGGCAA	172–193	196

Some degenerate base symbols have been used (R = A or G, N = A, T, G or C).