Figure 3.

Neurons in the neonatal cortex are transiently sensitive to elevated ambient glutamate. a, Example traces from voltage-clamped, cortical L5Ps held at 40 mV during application of APV (50 μm). Dashed lines indicate baseline and final holding currents. Calibration: x = 60 s, y = 25 pA. b, APV-sensitive holding currents (baseline − APV) in individual cells, normalized to capacitance on a cell-by-cell basis. Line indicates spline fit. c, Data in b, binned by age at P3–P6 (n = 11 cells/4 animals), P7–P8 (n = 9 cells/3 animals), and P10⁺ (n = 7 cells/5 animals). *p = 0.014 (one-way ANOVA with Tukey post hoc test). **p = 0.0010 (one-way ANOVA with Tukey post hoc test). Data are mean ± SEM.