Skip to main content
. 2019 May 8;39(19):3600–3610. doi: 10.1523/JNEUROSCI.3101-18.2019

Figure 1.

Figure 1.

Viral overexpression of GIRK channel subunits in VTA DA neurons. A, DATCre(+) mice were given intra-VTA infusion of Cre-dependent control, GIRK2, or GIRK3 vector. Following viral injection (3 w), mice were processed for either immunohistochemistry or slice electrophysiology. B, Control virus expression in the VTA of DATCre(+) mice. In the region of viral infusion, the percentage of GFP+ neurons that were also dopaminergic (TH+) was calculated to determine viral fidelity in control (C), GIRK2 (D), and GIRK3 (E) viral conditions. F, The overexpression of GIRK2 (n = 7 cells from 3 mice, 547 ± 111 pA) enhanced GABABR currents compared with cells expressing a control virus (n = 6 cells from 2 mice, 238 ± 24 pA; Mann–Whitney U test, U = 6, p = 0.035). G, The overexpression of GIRK3 (n = 8 cells from 5 mice, 33 ± 7 pA) diminished GABABR currents compared with cells expressing a control virus (n = 4 cells from 3 mice, 255 ± 44 pA; Mann–Whitney U test, U = 0, p = 0.004). *p < 0.05. **p < 0.01.