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. 2019 Feb 27;12(3):283–290. doi: 10.1111/cts.12613

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© 2019 The Authors. Clinical and Translational Science published by Wiley Periodicals, Inc. on behalf of the American Society for Clinical Pharmacology and Therapeutics. This article has been contributed to by US Government employees and their work is in the public domain in the USA.

This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.

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Depletion of CD20⁺ B cells does not affect nontarget cells. A known quantity of peripheral blood was collected, red blood cells lysed, and leukocytes stained with monoclonal antibodies. Counting beads were used to determine the total number of human leukocytes (white blood cells (WBCs)) and human T cells. Average yields of human WBCs/μL (a) and human T cells/μL (b) of blood are shown for each treatment group. No statistically significant differences were identified among treated vs. control groups.