The effects of dichloroacetate (DCA) treatment on alignment of collagen, mucoid accumulation, and ectopic mineralization within the tendon. The DCA group had significant improvement in alignment, as shown by decreases in circular standard deviation during (A) mechanical testing (n = 10) and (B) polarized light microscopy (n = 4). The DCA group had less glycosaminoglycans and less volume of mineralized materials, as evaluated by (C, D) alcian blue (AB) staining (n = 4) and (E, F) micro–computed tomography (μ-CT) analysis (n = 5). Scale bar is 125 mm for panel B. Values are presented as mean ± 1 SD.