Table 2. Screening of combinatorial drugs in macrophage model of M. tuberculosis infection.
Screening test | Iteration #1 | Iteration #2 |
Iteration #3A |
Iteration #3B |
Iteration #3C |
|
---|---|---|---|---|---|---|
Effect levelsa | 2 | 3 | 3 | 5 | 5 | 3 |
Test runsb | 150 | 102 | 155 | 75 | 75 | 82 |
Drugsc | AC | AC | AC | AC | AC | AC |
BDQ | BDQ | BDQ | BDQ | BDQ | BDQ | |
CFZ | CFZ | CFZ | CFZ | CFZ | CFZ | |
DLM | DLM | DLM | DLM | DLM | DLM | |
RIF | RIF | RIF | RIF | RIF | RIF | |
SQ109 | SQ109 | SQ109 | SQ109 | SQ109 | SQ109 | |
PA824 | PA824 | PA824 | PA824 | PA824 | ||
PZA | PZA | PZA | PZA | |||
EMB | EMB | EMB | ||||
PAS | PAS | |||||
PRS | PRO | |||||
CYS | ||||||
INH | ||||||
LZD | ||||||
MXF |
aScreening test was conducted at individual drug concentration that yielded 0% or 10% of the maximal inhibition to the IPTG-induced green fluorescence signal. Iteration #1 was conducted at individual drug concentration that gave 0% or 10% of the maximal inhibition level and one-half of the concentration that gave 10% of the maximal inhibition level. Iteration #2 was done at 0% or 15% effect levels and one-half of the concentration that gave 15% of the maximal inhibition level. Iterations #3A and 3B were conducted at 0% or 20% effect level and two-thirds, one-half or one-fourth of the concentration that gave 20% of the maximal inhibition level. Iteration #3C was conducted at 0% or 15% effect levels and one-half of the concentration that gave 15% of the maximal inhibition level.
bNumber of combinatorial drug test runs in each experiment.
cList of drugs tested at screening and each stage of iterations.