Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference |
Identifiers | Additional information |
|---|---|---|---|---|
| Genetic reagent(D. melanogaster) | w1118 | |||
| Genetic reagent(D. melanogaster) | zyd1 | Melom and Littleton, 2013 | zyd | |
| Genetic reagent(D. melanogaster) | repo-gal4 | Lee and Jones, 2005 | ||
| Genetic reagent(D. melanogaster) | NP2222-gal4 | Hayashi et al., 2002 | RRID:DGGR_112830 | |
| Genetic reagent(D. melanogaster) | GMR5H02-gal4 | RRID:BDSC_45784 | ||
| Genetic reagent(D. melanogaster) | GMRH02-lexA | Gift of Gerald M Rubin | ||
| Genetic reagent (D. melanogaster) | UAS-cam-RNAi | RRID:BDSC_34609 | CamRNAi | |
| Genetic reagent (D. melanogaster) | UAS-CanB-RNAi | RRID:BDSC_27307 | CanBRNAi#1 | |
| Genetic reagent (D. melanogaster) | UAS-CanB-RNAi | VDRC 21611 RRID:FlyBase_FBst0454139 |
CanBRNAi#2 | |
| Genetic reagent (D. melanogaster) | UAS-CanB-RNAi | VDRC 52390 RRID:FlyBase_FBst0469806 |
CanBRNAi#3 | |
| Genetic reagent (D. melanogaster) | UAS-CanB2-RNAi | RRID:BDSC_27270 | CanB2RNAi#1 | |
| Genetic reagent (D. melanogaster) | UAS-CanB2-RNAi | VDRC 104370 | CanB2RNAi#2 | |
| Genetic reagent (D. melanogaster) | UAS-CanB2-RNAi | VDRC 28764 RRID:FlyBase_FBst0457632 |
CanB2RNAi#3 | |
| Genetic reagent (D. melanogaster) | UAS-CanB2-RNAi | RRID:BDSC_38971 | CanB2RNAi#4 | |
| Genetic reagent (D. melanogaster) | UAS-Pp2B-14D-RNAi | RRID:BDSC_25929 | Pp2B-14DRNAi#1 | |
| Genetic reagent (D. melanogaster) | UAS-Pp2B-14D-RNAi | RRID:BDSC_40872 | Pp2B-14DRNAi#2 | |
| Genetic reagent (D. melanogaster) | UAS-CanA-14F-RNAi | RRID:BDSC_38966 | CanA-14FRNAi#1 | |
| Genetic reagent (D. melanogaster) | UAS-CanA-14F-RNAi | VDRC 30105 RRID:FlyBase_FBst0458337 |
CanA-14FRNAi#2 | |
| Genetic reagent (D. melanogaster) | UAS-cac-RNAi | VDRC 104168 | cacRNAi | |
| Genetic reagent (D. melanogaster) | UAS-sand-RNAi | VDRC 47977 RRID:FlyBase_FBst0467653 |
sandRNAi#1 | |
| Genetic reagent (D. melanogaster) | UAS-sand-RNAi | RRID:BDSC_25853 | sandRNAi#2 | |
| Genetic reagent (D. melanogaster) | UAS-Rab5-RNAi | RRID:BDSC_34832 | Rab5RNAi | |
| Genetic reagent (D. melanogaster) | UAS-Rab5.S43N | RRID:BDSC_42703
RRID:BDSC_42704 |
Rab5DN | |
| Genetic reagent (D. melanogaster) | UAS-zyd-RNAi | VDRC 40987 RRID:FlyBase_FBst0463881 |
zydRNAi | |
| Genetic reagent (D. melanogaster) | UAS-EKO[+] | White et al., 2001 | RRID:BDSC_40973 | |
| Genetic reagent (D. melanogaster) | CalexA | Masuyama et al., 2012 | RRID:BDSC_66542 | |
| Genetic reagent (D. melanogaster) | Tub-gal80ts | RRID:BDSC_7018
RRID:BDSC_7019 |
||
| Genetic reagent (D. melanogaster) | UAS- Pp2B-14FH217Q | Takeo et al., 2012 | RRID:DGGR_109869 | |
| Genetic reagent (D. melanogaster) | CanB2KO | Nakai et al., 2011 | Gift of Toshiro Aigaki | |
| Genetic reagent (D. melanogaster) | UASc-sand:GFP | Generated in this study | ||
| Antibody | Rabbit polyclonal anti-GFP | ThermoFisher | #A11122 RRID:AB_221569 |
Western blot, 1:5000 |
| Antibody | Mouse monoclonal anti-syx1A | DSHB | #8C3 RRID:AB_528484 |
Western blot, 1:500 |
| Antibody | Mouse monoclonal anti-Tubulin | Sigma Aldrich | #T5168 RRID:AB_477579 |
Western blot, 1:1,000,000 |
| Antibody | Rabbit polyclonal anti-cleaved-Drosophila DCP1 | Cell Signaling | #9578 RRID:AB_2721060 |
Western blot, 1:250 |
| Antibody | Mouse monoclonal anti-repo | DSHB | #8D12 RRID:AB_528448 |
IF, 1:25 |
| Antibody | Rat monoclonal anti-elav | DSHB | #7E8A RRID:AB_2800446 |
IF, 1:50 |
| Antibody | Rabbit polyclonal antiGFP-488 | Invitrogen | #A21311 RRID:AB_221477 |
IF, 1:500 |
| Antibody | Goat polyclonal anti-Mouse405 | Life technologies | #A31553 RRID:AB_221604 |
IF, 1:3000 |
| Antibody | Goat polyclonal anti-Rat555 | Invitrogen | #A21434 RRID:AB_2535855 |
IF, 1:3000 |
| Antibody | IRDye680LT Goat anti-Mouse IgG Secondary Antibody |
LI-COR | #926–68020 RRID:AB_10706161 |
Western blot, 1:3000 |
| Antibody | IRDye800CW Goat anti-Rabbit IgG Secondary Antibody |
LI-COR | #926–32211 RRID:AB_621843 |
Western blot, 1:3000 |
| Chemical compound, drug | CyclosporinA | Sigma Aldrich | #30024 | 1 mM |
| Chemical compound, drug | FK506 | InvivoGen | tlrl-fk5 | 1 mM |
| Enzyme | Alkaline Phospatase | Promega | #M282A |
*For a complete list of all RNAi stocks used in this study, see Supplementary file 2.