Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference |
Identifiers | Additional information |
|---|---|---|---|---|
| Strain, strain background (M.Muscularus) | Vglut2-Cre (Slc17a6-Cre) | The Jackson Laboratory | RRID:IMSR_JAX:028863 | |
| Strain, strain background (M.Muscularus) |
Chat-Chr2 (ChAT-ChR2-EYFP Line 6) | The Jackson Laboratory | RRID:IMSR_JAX:014546 | |
| Strain, strain background (Adeno-Associated Virus Serotype 2/9) | AAV-Ef1a-Flex-hChR2(H134)-EYFP-WPRE-hGHpA (2/9); Flex-Chr2 | This Paper; Neurocconnectivity Core at the Jan and Dan Duncan Neurological Research Institute | Plasmid subcloned from RRID:Addgene_26973 | |
| Strain, strain background (Adeno-Associated Virus Serotype 2/9) | AAV-Ef1a-Flex-hM4Di-mCherry (2/9); Flex-hM4Di-mCherry | This Paper; Neurocconnectivity Core at the Jan and Dan Duncan Neurological Research Institute | Plasmid subcloned from RRD:Addgene_44362 | |
| Strain, strain background (Adeno-Associated Virus Serotype 2/DJ8) | AAV-Ef1a-Flex-GCaMP6m-WPRE-hGHpA (DJ8); Flex-GCaMP6m | This Paper; Neurocconnectivity Core at the Jan and Dan Duncan Neurological Research Institute | Plasmid subcloned from RRID:Addgene_100839 |
|
| Strain, strain background (Adeno-Associated Virus Serotype 2/DJ8) | AAV-Ef1a-Flex-eGFP-p2a-mNaChBac-WPRE-hGHpA (DJ8); Flex-mNaChBac; mNaChBac | This Paper; Neurocconnectivity Core at the Jan and Dan Duncan Neurological Research Institute | Plasmid subcloned from plasmid gift from Dr. Mingshan Xue (mxue@bcm.edu) | |
| Strain, strain background (Adeno-Associated Virus Serotype 2/DJ8) | AAV-Ef1a-Flex-Synaptophysin::eGFP-WPRE-hGHpA (DJ8); Flex-Synaptophysin | This Paper; Neurocconnectivity Core at the Jan and Dan Duncan Neurological Research Institute | Plasmid subcloned from RRID:Addgene_73816 | |
| Strain, strain background (Adeno-Associated Virus Serotype 2/DJ8) | AAV-Ef1a-Flex-mRuby2 (DJ8); Flex-mRuby2 | This Paper; Neurocconnectivity Core at the Jan and Dan Duncan Neurological Research Institute | Plasmid subcloned from Addgene:40260 | |
| Recombinant DNA reagent (plasmid) | pAAV-Ef1a-Flex-hChR2(H134)-EYFP-WPRE-hGHpA | This paper | RRID:Addgene_26973 | |
| Recombinant DNA reagent (plasmid) | pAAV-Ef1a-Flex-hM4Di-mCherry | This paper | RRID:Addgene_44362 | |
| Recombinant DNA reagent (plasmid) | pAAV-Ef1a-Flex-GCaMP6m-WPRE-hGHpA | This paper | RRID:Addgene_100839 | |
| Recombinant DNA reagent (plasmid) | pAAV-Ef1a-Flex-eGFP-p2a-mNaChBac-WPRE-hGHpA | Plasmid subcloned from plasmid gift from Dr. Mingshan Xue (mxue@bcm.edu) | ||
| Recombinant DNA reagent (plasmid) | pAAV-Ef1a-Flex-Synaptophysin::eGFP-WPRE-hGHpA | This paper | RRID:Addgene_73816 | |
| Recombinant DNA reagent (plasmid) | pAAV-Ef1a-Flex-mRuby2 | This Paper | RRID:Addgene_40260 | |
| Antibody | anti-ChAT (goat monoclonal) | Millipore | Millipore Cat# AB144P; RRID:AB_2079751 | (1: 500 dilution) |
| Antibody | anti-cFos (rabbit polyclonol) | Abcam | Abcam Cat# ab190289; RRID:AB_2737414 | (1:1000 dilution) |
| Chemical compound, drug | CNO (clozapine-n-oxide) | Tocris 4963 | ||
| Commercial assay or kit | Mouse/Rat T4 Elisa | Calbiotech, Inc | T4044T-100 | |
| Commercial assay or kit | Active Ghrelin ELISA | Millipore | EZRGRA-90K | |
| Software, algorithm |
Doric Neuroscience Studio | Doric Lenses | ||
| Software, algorithm | MATLAB | Mathworks | RRID:SCR_001622 | |
| Software, algorithm | Optimouse | PMID:28506280 | ||
| Software, algorithm | Prism 8 | Graphpad | GraphPad Prism, RRID:SCR_002798 | |
| Software, algorithm | Rodent Liquid diet | Test Diet | Test Diet:101 5LD1 | |
| Other (Mouse Liquid Diet) | Synapse | Tucker-Davis Technologies | Tucker-Davis Technologies; RRID:SCR_006495 | |
| Other (GRIN Lens) | Grin lens | Doric Lenses | Doric:SICL_E_500_80 |