Skip to main content
. Author manuscript; available in PMC: 2019 Jun 4.
Published in final edited form as: Dev Cell. 2018 Jun 4;45(5):651–660.e4. doi: 10.1016/j.devcel.2018.05.010

Fig.4. Positional identities in the nephron are specified by gradual recruitment of progenitor cells.

Fig.4

(A) Immunofluorescent analysis of nephron-segment identity markers in (A) SSBs where fates are demarked as follows: D: distal tubule, L: loop of Henle anlagen, M: medial segment, Pt: proximal tubule, Pe: parietal epithelium, Po: podocytes. (B) emerging proximal-distal polarities at 2 positions (1 and 2) during PTA-RV-CSB stages. Scale bars 10 μm. Immunofluorescent stains as indicated. Nephron development stage indicated above fields in B. (C) Top: Schematic model for progressive recruitment of NPCs over time and sequential cell fate acquisition. Bottom: Specification of cell-fates along cumulative time as indicated by pseudotime. PTA: pretubular aggregate, RV: renal vesicle, SSB: S-shaped body nephron, UB: ureteric bud, NPC: nephron progenitor, LOH: loop of Henle analage, RC: renal corpuscle precursor.