Association of NMIIA with the RBC membrane skeleton in
MYH9-RD patients. (A-C) RBC Mg++ ghosts from
normal donor shipped controls and MYH9-RD motor (A),
coiled-coil rod (B), and non-helical tail (C) domain patients were extracted in
Triton X-100 buffer followed by SDS-PAGE and immunoblotting for NMIIA heavy
chain (top) and actin (bottom) in the supernatant (S) and Triton-insoluble
membrane skeleton pellet (P) fractions. Each pair of lanes represents a unique
normal donor or patient, in the same order as in Figure S9. (D) Quantification of %
NMIIA in the membrane skeleton pellet fraction, comparing normal shipped
controls (dot plot) with individual motor domain patients (black bars),
coiled-coil rod domain patients (dark gray bars), and non-helical tail domain
patients (light gray bars). Each dot in the dot plot and each bar in the bar
graph represents one technical replicate of each sample. (E) Quantification in
(D) grouped by normal donor shipped controls, motor domain patients, and
coiled-coil rod/non-helical tail domain patients. One-way ANOVA followed by
Tukey’s multiple comparisons test, Controls vs. Motor, p = 0.0039; Motor
vs. Rod/Tail, p = 0.0276. In dot plots, lines represent mean ± S.D.