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. Author manuscript; available in PMC: 2019 May 10.
Published in final edited form as: ACS Infect Dis. 2018 Dec 19;5(5):759–768. doi: 10.1021/acsinfecdis.8b00269

Fig. 4. BA-53038B inhibited the assembly of pgRNA-containing nucleocapsids.

Fig. 4.

AML12HBV10 cells were cultured in the absence of tet and treated with the indicated concentrations of BA-53038B, 5 μM of DVR-23, 2 μM of Bay 41–4109 or 1 μM of ETV. Two days post treatment, total HBV RNA (A) and encapsidated pgRNA (B) were detected by Northern blot hybridization with a 32P-labeled riboprobe to detect minus-strand HBV DNA. 28S and 18S rRNA served as loading controls. (C) Cytoplasmic HBV capsids were resolved by a 1.8 % agarose gel electrophoresis followed by detection of capsid with antibody against core protein and HBV DNA, as described in Fig.2A legend. (D) HBV DNA replication intermediates were detected by Southern blot hybridization. rcDNA, dslDNA and ssDNA are relaxed circular, double-stranded linear and single-stranded forms of HBV DNA, respectively.