Figure 2.

Examples of Day 35 fetal genotyping using PCR amplification to detect editing of sialoadhesin (SIGLEC1/ SN/ CD169). Donor cell lines used in early gestation survival experiments were homozygous or heterozygous SIGLEC1 knockouts or had no editing (wild type). (a) Example of litter from heterozygous knockout and wild type cell lines used in somatic cell nuclear transfer. Corresponds to the litter in the last row of Table 2. (b) Example of litter from heterozygous and homozygous knockout cell lines used in somatic cell nuclear transfer. Corresponds to the litter in the first row of Table 2. PCR: polymerase chain reaction