Table 3.
mRNA expression levels in control and IUGR fetal sheep LV and RV aged ~134 dGA.
| Relative Expression Levels | Interaction | Main Effects | |||||
|---|---|---|---|---|---|---|---|
| RV | LV | Treatment | Ventricle | ||||
| Control (n=3) |
IUGR (n=7) |
Control (n=7) |
IUGR (n=13) |
P-value | P-value | P-value | |
| Reference genes | |||||||
| RPL32 | 0.99±0.22 | 0.93±0.18 | 1.00±0.04 | 0.98±0.25 | ns | ns | ns |
| RPL37A | 1.01±0.31 | 1.03±0.17 | 1.00±0.16 | 1.08±0.27 | ns | ns | ns |
| MLF2 | 1.03±0.15 | 1.05±0.24 | 1.00±0.18 | 0.92±0.28 | ns | ns | ns |
| Cyclins | |||||||
| Cyclin A2 | 1.28±0.55 | 0.67±0.23 | 1.00±0.51 | 0.57±0.23 | ns | 0.0014 | ns |
| Cyclin B1 | 1.98±1.18 | 0.74±0.23 | 1.00±0.44 | 0.67±0.33 | 0.0306 | ||
| Cyclin B2 | 1.61±0.88 | 0.74±0.26 | 1.00±0.27 | 0.72±0.23 | ns | 0.0006 | 0.0381 |
| Cyclin D1 | 1.15±0.16 | 1.16±0.22 | 1.00±0.23 | 1.13±0.34 | ns | ns | ns |
| Cyclin D2 | 1.87±0.24 | 1.50±0.30 | 1.00±0.20 | 1.02±0.16 | 0.043 | ||
| Cyclin E2 | 1.03±0.27 | 0.94±0.15 | 1.00±0.21 | 0.82±0.25 | ns | ns | ns |
| Cyclin dependent kinases | |||||||
| CDK1 | 1.37±0.57 | 0.86±0.29 | 1.00±0.35 | 0.62±0.37 | ns | 0.009 | ns |
| CDK4 | 1.02±0.13 | 1.10±0.21 | 1.00±0.15 | 0.94±0.19 | ns | ns | ns |
| CDK6 | 1.40±0.07 | 1.14±0.16 | 1.00±0.14 | 0.97±0.09 | 0.0345 | ||
| Cyclin dependent kinase inhibitors | |||||||
| CDKN2C (p18) | 1.12±0.19 | 1.06±0.19 | 1.00±0.18 | 0.80±0.22 | ns | ns | 0.0348 |
| CDKN1A (p21) | 0.49±0.15 | 0.67±0.18 | 1.00±0.42 | 0.91±0.26 | ns | ns | 0.0041 |
| CDKN1B (p27) | 0.92±0.03 | 1.23±0.35 | 1.00±0.11 | 0.99±0.25 | ns | ns | ns |
| Cell cycle effectors and checkpoint genes | |||||||
| MCM6 | 0.97±0.21 | 0.99±0.15 | 1.00±0.20 | 0.87±0.19 | ns | ns | ns |
| PLK1 | 1.47±0.99 | 0.78±0.28 | 1.00±0.78 | 0.70±0.30 | ns | 0.0299 | ns |
| CDC20 | 1.26±0.56 | 0.84±0.22 | 1.00±0.34 | 0.74±0.22 | ns | 0.0101 | ns |
| MAD2 | 1.23±0.40 | 0.87±0.21 | 1.00±0.26 | 0.70±0.32 | ns | 0.0134 | ns |
| BUB1 | 2.20±1.49 | 0.48±0.51 | 1.00±0.69 | 0.38±0.48 | ns | 0.0004 | 0.0303 |
| ESPL1 | 1.00±0.56 | 0.70±0.30 | 1.00±0.63 | 0.54±0.18 | ns | 0.0251 | ns |
| CHK1 | 1.09±0.21 | 1.10±0.31 | 1.00±0.25 | 0.83±0.38 | ns | ns | ns |
| DBF4 | 1.50±0.47 | 0.79±0.14 | 1.00±0.36 | 0.83±0.29 | 0.0398 | ||
| YWHAZ | 1.34±0.19 | 1.13±0.19 | 1.00±0.14 | 0.93±0.19 | ns | ns | 0.0015 |
| Transcription factors | |||||||
| TFDP1 | 0.96±0.10 | 0.94±0.14 | 1.00±0.09 | 0.94±0.16 | ns | ns | ns |
| Meis1 | 1.21±0.11 | 1.04±0.32 | 1.00±0.19 | 1.07±0.28 | ns | ns | ns |
Groups compared by 2-way ANOVA.
*
Different from ipsilateral control by Holm-Sidak test (P<0.05). Data are shown as mean±standard deviation.