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. 2019 May 10;9:7256. doi: 10.1038/s41598-019-43703-8

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Sequence similarities of NSP-like gene family members in Pieris spp. acquired from RNA-seq analysis. The tree is based on amino acid sequences and was generated using the Maximum Likelihood Method. Values shown at each node are Bootstrap values. Reference sequences from GenBank are provided with accession numbers. According to the RNA-seq data analysis, all four Pieris species encode for NSP, MA and SDMA proteins.