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. 2019 May 10;9:7255. doi: 10.1038/s41598-019-43561-4

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Tubulation of GSB membrane by Dyn1. (A) Representative images showing tubulation (indicated by arrows) induced by Dyn1 (0.5 µM) on GSB (left) versus GUV (right). The lipid composition in both cases was DOPC:DOPE:DOPS:PI(4,5)P₂:Chol:RhPE 58:10:15:2:30:1 mol%. RhPE fluorescence is shown. Scale bar 4 μm. (B) Inwards tubulation of a GSB membrane upon microinjection of Dyn1 (0.5 μM) into the GSB lumen. Overlaid bright field/fluorescence image (1) shows the experimental configuration during piercing of the GSB membrane by the injection pipette. Development of the inward membrane tubulation is shown in images (2) and (3). The lipid composition used was DOPC:DOPE:DOPS:PI(4,5)P₂:Chol:RhPE 27:24:15:3:30:1 mol%. Scale bar 15 μm.