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. 2019 May 10;9:7248. doi: 10.1038/s41598-019-43493-z

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Quantification of Y1R co-localization. Quantification of colocalization in medial and central dorsal horn of (A) Calbindin-ir, (B) Calretenin-ir, (C) Pax2-ir, and (D) PKCγ-ir with Y1R-ir. The relative distribution of subpopulations of Y1R-expressing neurons as determined by % colocalizations can be seen in (E,F). N = 3 rats per antibody with N = 3 transverse sections averaged per animal.