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Phenotypic characterization of non-differentiated HLSC (HLSC) and differentiated HLCS-ILS at different times of culture (7, 14 and 21 days). a The expression of human Ngn3, PDX1, C-peptide and glucagon were analyzed by Western Blot. b Representative micrographs of immunofluorescence and percentage of different cell subpopulations expressing insulin alone, insulin and glucagon and negative for both hormones
